High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism
Table 1
Comparison of intracellular metabolites levels between the control and HG groups.
Number
Metabolites
CON
HG
1
Isoleucine
0.56
1.06 ± 0.03
0.91 ± 0.01
2
Valine
0.87
1.65 ± 0.08
1.42 ± 0.03
3
Fucose
0.46
4.34 ± 0.26
4.70 ± 0.10
4
Alanine
0.95
13.77 ± 0.42
17.94 ± 0.42
5
Acetate
0.20
4.21 ± 0.19
4.43 ± 0.37
6
N-Acetylglutamate
0.21
4.52 ± 0.28
4.57 ± 0.20
7
Succinylacetone
0.56
2.19 ± 0.06
2.19 ± 0.05
8
Glutamate
0.92
38.69 ± 0.72
44.36 ± 0.58
9
Succinate
0.62
2.60 ± 0.16
3.03 ± 0.07
10
Glutamine
0.64
25.52 ± 0.68
26.47 ± 0.54
11
Methionine
0.38
4.35 ± 0.12
4.25 ± 0.08
12
Dimethylamine
0.71
0.90 ± 0.04
0.72 ± 0.03
13
Dimethylglycine
0.53
0.79 ± 0.04
0.63 ± 0.03
14
Aspartate
0.70
3.74 ± 0.17
3.15 ± 0.12
15
Creatine phosphate
0.54
18.63 ± 0.47
21.42 ± 0.43
16
Creatine
0.37
14.89 ± 0.87
12.74 ± 0.37
17
Phenylalanine
0.88
15.46 ± 0.33
17.33 ± 0.36
18
Choline
0.88
26.67 ± 0.68
31.04 ± 0.48
19
Myoinositol
0.98
47.27 ± 1.26
61.02 ± 1.22
20
Lactate
0.72
22.83 ± 0.63
19.87 ± 0.15
21
3-Methylhistidine
0.55
1.07 ± 0.06
0.88 ± 0.05
22
Formate
0.32
0.54 ± 0.02
0.44 ± 0.02
The absolute value of correlation coefficient obtained from PLS-DA; control group (25 mM glucose); high glucose group (75 mM glucose). Significant level: , , .
