High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A

Figure 1

TcdA induces the release of HMGB1 from CT26 cells. (a) CT26 cells were treated with different concentrations of TcdA for 4 h, and the rate of cell rounding was calculated. (b) CT26 cells were exposed to the medium (cell control) or the TcdA for 4 h. The percentage of cells affected (cell rounding) was observed under a phase-contrast microscope. (c) CT26 cells were exposed to 10 ng/mL TcdA for the indicated time intervals, and HMGB1 levels in the culture medium were detected by western blot analysis using BSA as a loading control. .

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