Review Article

Avian Mycobacteriosis: Still Existing Threat to Humans

Table 4

The most frequent sequencing targets used for identification of nontuberculous mycobacteria.

Target geneNamePrimer typeSequence Length of the product (bp)Reference

16S rRNA16S27f
16S907r
Forward
Reverse
AGAGTTTGATCMTGGCTCAG
CCGTCAATTCMTTTRAGTTT
921Harmsen et al., 2003 [12]

hsp65Tb11
Tb12
Forward
Reverse
ACCAACGATGGTGTGTCCAT
CGGATCACTCGTGAACGCTA
441Telenti et al., 1993 [13]

ITS16S-1511f
23S-23r
Forward
Reverse
AAGTCGTAACAAGGTARCCG
TCGCCAAGGCATCCACC
Approx. 380Harmsen et al., 2003 [12]

rpoBMyco-F
Myco-R
Forward
Reverse
GGCAAGGTCACCCCGAAGGG
AGCGGCTGCTGGGTGATCATC
764Adékambi et al., 2003 [14]

Amplicon size is not uniform due to the 16S-23S spacer length variability.