Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1α

Figure 5

HIF-1α is required for ID1 induction in the H/R model. HK-2 cells were transiently transfected with specific siRNA for HIF-1α or siRNA for negative control and then subjected to H/R. (a) In cells transfected with HIF-1α siRNA, the expression of HIF-1α was markedly inhibited and ID1 was suppressed, although not remarkable. β-actin served as the loading control. A representative blot from 3 independent experiments is shown. (b, c) In cells transfected with HIF-1α siRNA, relative mRNA levels of HIF-1α were efficiently reduced. Relative mRNA levels of ID1 were decreased significantly at the prehypoxia, 12-hour and 24-hour time points for hypoxia, and after 12 hours of reoxygenation. β-actin mRNA was used as the internal control (). compared with the negative control (scramble). ID1, inhibitor of DNA binding 1; HIF-1α, hypoxia-inducible factor-1 alpha; H/R, hypoxia-reoxygenation.

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