Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1α

Figure 7

Twist is induced in the H/R model and is regulated by HIF-1α and ID1. (a) Western blot analysis of twist expression in HK-2 cells during H/R. β-actin was examined as the loading control. A representative blot from 3 independent experiments is shown. (b) In cells transfected with HIF-1α siRNA, and relative mRNA levels of twist were significantly reduced after 24 hours of hypoxia. β-actin mRNA served as the internal control (). compared with the negative control (scramble). (c) In cells transfected with ID1 siRNA, relative mRNA levels of twist were significantly reduced after 24 hours of hypoxia. β-actin mRNA served as the internal control (). compared with the negative control (scramble). ID1, inhibitor of DNA binding 1; HIF-1α, hypoxia-inducible factor-1 alpha; H/R, hypoxia-reoxygenation.

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