Evaluation of Lethal Giant Larvae as a Schistosomiasis Vaccine Candidate

Figure 2

Expression and purification of rSjLGL in E. coli. Cell extracts and fractions from E. coli BL21 (DE3) cells that were transformed with the pET28-Sjlgl vector were separated by 12% SDS-PAGE. Lane M: size markers. Lane 1: total extract of a clone before induction. Lanes 2, 3, and 4: total extracts of a clone 2, 4, and 6 h after induction with 1 mM IPTG. Lanes 5 and 6: western blot using a polyclonal anti-rSjLGL antibody (lane 5: antigen (total extract of a clone before IPTG induction); lane 6: total extract of a clone after 5 h of IPTG induction; lane 7: protein purified from the supernatant by passage through Ni-NTA His-Bind Resin).