TY - JOUR A2 - Arciola, Carla R. AU - Dakheel, Khulood Hamid AU - Abdul Rahim, Raha AU - Neela, Vasantha Kumari AU - Al-Obaidi, Jameel R. AU - Hun, Tan Geok AU - Yusoff, Khatijah PY - 2016 DA - 2016/12/18 TI - Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion SP - 4708425 VL - 2016 AB - Twenty-five methicillin-resistant Staphylococcus aureus (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype was spa type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested. SN - 2314-6133 UR - https://doi.org/10.1155/2016/4708425 DO - 10.1155/2016/4708425 JF - BioMed Research International PB - Hindawi Publishing Corporation KW - ER -