A Randomized, Double-Blind, Placebo-Controlled Trial: The Efficacy of Multispecies Probiotic Supplementation in Alleviating Symptoms of Irritable Bowel Syndrome Associated with Constipation

Table 2

List of primers used in this study.

Probiotic	Primer code	Sequence ()	DNA region	Amplified length (bp)
L. rhamnosus	LraF	CTAGCGGGTGCGACTTTGTT	16S/23S IS	123 bp
	LraR	CAGCGGTTATGCGATGCGAA
L. plantarum	Lpl2F	CATTGGAACCGAACCAGTTG	16S/23S IS	203 bp
	Lpl2R	CGGTGTTCTCGGTTTCATTATG
B. animalis subsp. lactis	AnimF	GCACGGTTTTGTGGCTGG	pre16S	171 bp
	AnimR	GACCTGGGGGACACACTG
L. acidophilus	Lacid2F	GGGCAAATCACGAACGAGTA	pre16S	132 bp
	Lacid2R	CTTTGTTTTCGTTCGCTTCA
L. reuteri	Lreu2F	GTTGACGAAAGAATGAAATCCA	pre16S	118 bp
	Lreu2R	TCATGTCGTCAATCAGATGTCA