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BioMed Research International
Volume 2016, Article ID 4814702, 6 pages
http://dx.doi.org/10.1155/2016/4814702
Research Article

Differential Proteomics Analysis of Colonic Tissues in Patients of Slow Transit Constipation

Zhongnan Hospital of Wuhan University, Department of Colorectal & Anal Surgery, Clinical Center of Intestinal and Colorectal Diseases of Hubei Province, Key Laboratory of Intestinal & Colorectal Diseases of Hubei Province, Wuhan University, No. 169, Donghu Road, Wuchang District, Wuhan, Hubei 430071, China

Received 2 March 2016; Accepted 11 April 2016

Academic Editor: Terry K. Smith

Copyright © 2016 Songlin Wan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate and screen the different expression of proteins in STC and normal group with a comparative proteomic approach. Methods. Two-dimensional electrophoresis was applied to separate the proteins in specimens from both 5 STC patients and 5 normal controls. The proteins with statistically significant differential expression between two groups were identified by computer aided image analysis and matrix assisted laser desorption ionization tandem time of flight mass spectrometry (MALDI-TOF-MS). Results. A total of 239 protein spots were identified in the average gel of the normal control and 215 in patients with STC. A total of 197 protein spots were matched and the mean matching rate was 82%. There were 14 protein spots which were expressed with statistically significant differences from others. Of those 14 protein spots, the expression of 12 spots increased markedly, while that of 2 spots decreased significantly. Conclusion. The proteomics expression in colonic specimens of STC patients is statistically significantly different from that of normal control, which may be associated with the pathogenesis of STC.