Research Article

RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism

Figure 4

Xyr1 functionally binds to the upstream regions of four genes downregulated in Δxyr1 on inducing medium. Binding of Xyr1 to the promoter regions of the intracellular β-glucosidase-encoding gene bgl2 (a), the nonenzymatic cellulose-attacking enzyme-encoding gene cip1 (b), the putative MFS lactose transporter-encoding gene lp (c), and the nmr-like gene (d). The amounts of purified Xyr1 (μM) used were as indicated and about 10 ng of Cy5-labeled probes was added to each reaction. The specificity of shifts was verified by adding 100-fold excess unlabeled specific (S) and nonspecific (NS) competitor DNA. Purified GST (1 μM) was used as the negative control to exclude nonspecific binding by GST.
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