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BioMed Research International
Volume 2016, Article ID 4874809, 11 pages
http://dx.doi.org/10.1155/2016/4874809
Research Article

Vitamin A Inhibits Development of Dextran Sulfate Sodium-Induced Colitis and Colon Cancer in a Mouse Model

1Department of Pathology, School of Medicine, Kitasato University, Sagamihara, Kanagawa 252-0374, Japan
2Research Center for Biological Imaging, School of Medicine, Kitasato University, Sagamihara, Kanagawa 252-0374, Japan
3Laboratory of Immunology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa 769-2193, Japan
4Institute of Neuroscience, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa 769-2193, Japan

Received 25 January 2016; Revised 30 March 2016; Accepted 12 April 2016

Academic Editor: Andrea Vecchione

Copyright © 2016 Isao Okayasu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary FIGURE 1. Intracellular lipid droplets in a stellate cell of the liver. No lipid droplets were observed in a hepatic stellate cell of a vitamin A-deficient mouse (right) in contrast to detection of lipid droplets (arrow) in that of a vitamin A-supplemented mouse (left) as assessed by electron microscopy. Black bar indicates 1 μm in length.

Supplementary FIGURE 2. Inflammatory lesions in DSS colitis. (a) Focal inflammatory cell infiltration including polymorphonuclear leucocytes (colitis score 1). (b) Inflammatory granulation with crypt abscess (colitis score 2). (c) Inflammatory granulation with gland dropout (colitis score 2). (d) Mucosal erosion (ulceration) with inflammatory granulation (colitis score 3).

Supplementary TABLE 1. (a) Acute colitis induced with DSS drinking for 7 days (on day 8) (M±SD). (b) Acute colitis induced with DSS drinking for 12 days (on day 12) (M±SD). (c) Long term-colitis induced with DSS drinking for 7 days (on day 43) (M±SD).

Supplementary TABLE 2. Colorectal neoplasia and colitis induced with a combination of azoxymetane preinjection and DSS drinking for 7 days (on day 28) (M±SD).

  1. Supplementary Material