Research Article
Downregulation of ADAMTS8 by DNA Hypermethylation in Gastric Cancer and Its Clinical Significance
Figure 2
The ADAMTS8 methylation status in gastric cancer cells and gastric tumor samples. (a) Schematic diagram of CpG islands (CGI) in ADAMTS8. CGI across ADAMTS8 promoter and exon 1. The vertical bars indicate the location of each of the CpG sites and the thick line indicates the location of the MSP primers and BGS primers. (b) Methylation of ADAMTS8 in GES1 and four gastric cancer cell lines by MSP analysis. (c) Methylation of ADAMTS8 in paired primary gastric tumors and corresponding nontumor tissues by MSP analysis. U: unmethylated; M: methylated; IVD: positive control; H2O: negative control. (d) Association between ADAMTS8 mRNA expression level and ADAMTS8 methylation (). (e) ADAMTS8 CpG methylation status in GES1 and four gastric cancer cells by BGS. (f) ADAMTS8 CpG methylation status in two matched primary gastric tumors and corresponding nontumor tissues by BGS.
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