Research Article
Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection
Table 2
Result comparison of real-time PCR with HC2 and fragment analysis.
| Real-time PCR | HC2 | Fragment analysis | Total | Positive | Negative | Error | Type 16 | Type 18 | HRC | Type 16 & HRC | Type 18 & HRC | Types 16 & 18 | Not tested | Not detected |
| Positive | Type 16 | 40 (72.7%) | 14 | 1 | 39 (70.9%) | 0 | 1 | 8 | 0 | 0 | 7 | 0 | 55 | Type 18 | 22 (66.7%) | 10 | 1 | 0 | 22 (66.7%) | 1 | 0 | 2 | 0 | 2 | 6 | 33 | HRC | 395 (82.0%) | 86 | 1 | 3 | 0 | 375 (77.8%) | 12 | 1 | 0 | 38 | 53 | 482 | Type 16 & HRC | 12 (100%) | 0 | 0 | 4 | 0 | 1 | 7 (58.3%) | 0 | 0 | 0 | 0 | 12 | Type 18 & HRC | 9 (90.0%) | 1 | 0 | 0 | 2 | 2 | 0 | 5 (50.0%) | 0 | 1 | 0 | 10 | Types 16 & 18 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | Total | 479 | 111 | 3 | 46 | 24 | 380 | 27 | 8 | 1 | 48 | 59 | 593 |
| NDBA | Type 16 | 4 (21.1%) | 15 | 0 | 8 (42.1%) | 0 | 6 | 0 | 0 | 0 | 0 | 5 | 19 | Type 18 | 8 (38.1%) | 13 | 0 | 1 | 1 (4.8%) | 1 | 0 | 2 | 0 | 2 | 14 | 21 | HRC | 34 (20.4%) | 127 | 6 | 2 | 0 | 58 (34.7%) | 2 | 0 | 0 | 2 | 103 | 167 | Type 16 & HRC | 0 | 9 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 6 | 9 | Type 18 & HRC | 0 | 5 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 5 | Types 16 & 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | Total | 46 | 169 | 6 | 13 | 1 | 68 | 3 | 2 | 0 | 4 | 130 | 221 |
|
|
High risk common.
|