Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection

Table 5

Comparison of four cut-off values in real-time PCR to fragment analysis for detection of high risk HPV ().

HPV type	Cut-off	Number of specimens with results				% Sensitivity	% Specificity
		TP	FP	TN	FN
16	31	54	2	671	36	60.0	99.7
	32	59	2	671	31	65.6	99.7
	33	62	2	671	28	68.9	99.7
	34	65	6	667	25	72.2	99.1
18	31	31	7	721	4	88.6	99.0
	32	32	9	713	3	91.4	98.8
	33	33	10	718	2	94.3	98.6
	34	33	12	716	2	94.3	98.4
HRC	31	369	49	218	127	74.4	81.6
	32	403	62	205	93	81.3	76.8
	33	424	81	186	72	85.5	69.7
	34	447	107	160	49	90.1	59.9

High risk common.
True positive.
False positive.
True negative.
False negative.