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BioMed Research International
Volume 2016, Article ID 5173205, 11 pages
Research Article

HMBA Enhances Prostratin-Induced Activation of Latent HIV-1 via Suppressing the Expression of Negative Feedback Regulator A20/TNFAIP3 in NF-κB Signaling

1State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China
2Department of Genetics, Xuzhou Medical College, Xuzhou, Jiangsu 221000, China

Received 21 December 2015; Accepted 21 June 2016

Academic Editor: Marcelo A. Soares

Copyright © 2016 Duchu Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Prostratin did not further stimulate HMBA-induced Brd4 liberation from chromatin. Antagonizing the NF-κB signaling by BAY, an IKK inhibitor, could block HMBA and prostratin co-treatment (H+P)-induced transcriptional activation of HIV-LTR-Luc at both the initiation and elongation stages.

HMBA could enhance prostratin-induced degradation of IκBα, while MG-132 pre-treatment could block this stimulation-induced degradation.

  1. Supplementary Material