Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis

Table 1

Number of detected protein spots on the 2DE gel images.

Bile fluid treatment method ()	Number of protein spots
Untreated	39
2D Clean-Up kit	52
Acetone precipitation method	170
Acetone followed by 2D Clean-Up kit	558^#

Comparison between acetone followed by 2D Clean-Up kit and the other 3 treatment methods ().