Research Article

Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis

Table 2

Comparisons of total protein concentrations after different bile fluid sample treatment methods.

Bile fluid treatment method ( = 12) Concentration in μg/μL

Untreated0.797 ± 0.465
2D Clean-Up kit0.598 ± 0.213
Acetone precipitation method1.478 ± 0.415#
Acetone followed by 2D Clean-Up kit0.866 ± 0.331

Comparison between acetone followed by 2D Clean-Up kit and the other 3 treatment methods ().