Progressive Depletion of Rough Endoplasmic Reticulum in Epithelial Cells of the Small Intestine in Monosodium Glutamate Mice Model of Obesity

Figure 5

Representative light microscopy images showing the distribution of rough endoplasmic reticulum (rER) in small intestine mucosal epithelial cells. ((a) and (b)) High magnification views of Nissl-stained sections of small intestine from control mice and monosodium glutamate- (MSG-) induced obese mice, respectively. ((c) and (d)) High magnification views of rER-stained sections small intestine from control mice and MSG-induced obese mice, respectively. ((e) and (f)) High magnification views of Golgi apparatus-stained sections of small intestine from control mice and MSG-induced obese mice, respectively. (g) High magnification view of tight junction-stained sections of small intestine from control mice. ((h) and (i)) High magnification views of tight junction-stained sections of small intestine from MSG-induced obese mice. Arrowheads in (a) indicate intensely Nissl-stained regions in the cytoplasm. Double arrowheads in (b) indicate slightly or negative Nissl-stained regions in the cytoplasm. White arrows in (c) and (d) indicate rER. White arrows in (e) and (f) indicate Golgi apparatus. White arrows in (g) and (h) indicate tight junctions. All scale bars = 10 μm.

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