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BioMed Research International
Volume 2016, Article ID 5381956, 11 pages
http://dx.doi.org/10.1155/2016/5381956
Research Article

Relationship between Antibody Levels, IgG Binding to Plasmodium falciparum-Infected Erythrocytes, and Disease Outcome in Hospitalized Urban Malaria Patients from Dakar, Sénégal

1Service d’Immunologie FMPO, Université Cheikh Anta Diop de Dakar, Dakar, Senegal
2Unité d’Immunogénétique, Institut Pasteur de Dakar, Dakar, Senegal
3Service de Réanimation, Hôpital Principal de Dakar, Dakar, Senegal
4Unité d’Immunologie, Institut Pasteur de Dakar, Dakar, Senegal

Received 22 February 2016; Revised 6 May 2016; Accepted 24 May 2016

Academic Editor: Fernando A. Genta

Copyright © 2016 Babacar Mbengue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Management of clinical malaria requires the development of reliable diagnostic methods and efficient biomarkers for follow-up of patients. Protection is partly based on IgG responses to parasite antigens exposed at the surface of infected erythrocytes (iRBCs). These IgG responses appeared low during clinical infection, particularly in severe disease. Methods. We analyzed the IgG binding capacity to the surface of live erythrocytes infected by knob positive FCR3 strain. Sera from 69 cerebral malaria (CM) and 72 mild malaria (MM) cases were analyzed by ELISA for IgG responses to five antigens from iRBC and by flow cytometry for IgG binding as expressed in labeling index ratio (LIR). The relationship between IgG levels, LIR, parasitemia, age, and the clinical outcomes was evaluated. Results. We found a significant decrease of LIR in adult CM fatal cases compared to surviving patients (). In MM, LIRs were correlated to IgG anti-iRBC and anti-PfEMP3/5 levels. In CM, no correlation was found between LIR, IgG levels, and parasitemia. Conclusion. The IgG binding assay was able to discriminate outcome of cerebral malaria cases and it deserves further development as a potential functional-associated assay for symptomatic malaria analysis.