Altered Clock and Lipid Metabolism-Related Genes in Atherosclerotic Mice Kept with Abnormal Lighting Condition

Figure 3

Circadian expression of clock genes in liver of C57, ApoE-KO, and ApoE-KO LD/DL mice. We obtained the liver tissues of mice at time points of ZT0, ZT4, ZT8, ZT12, ZT16, and ZT20. Levels of mRNA were determined by quantitative real-time PCR. In the liver tissue, the four clock genes Bmal1, Per2, Cry1, and Rev-erb α exhibit significant 24 h circadian expression pattern in C57, ApoE-KO, and ApoE-KO LD/DL mice. The mesors of Bmal1 and Per2 were reduced in ApoE-KO LD/DL mice compared with those in C57 and ApoE-KO mice. The amplitudes of Cry1 in ApoE-KO mice and ApoE-KO LD/DL mice were decreased compared with those in C57 mice, and the amplitudes of Per2 and Rev-erb α in ApoE-KO LD/DL mice were attenuated compared with those in ApoE-KO mice. The acrophases of Bmal1, Cry1, and Per2 in ApoE-KO LD/DL mice were altered compared with those in C57 and ApoE-KO mice. The mRNA levels of clock genes were normalized to GAPDH mRNA ( for each group at every time point).