Altered Clock and Lipid Metabolism-Related Genes in Atherosclerotic Mice Kept with Abnormal Lighting Condition

Figure 5

Circadian expression of PPAR α, PPAR γ, ROR α, and RXR α in mouse liver tissues. We also identified the diurnal expression of lipid metabolism-associated genes (PPAR α, PPAR γ, ROR α, and RXR α) in liver at specific time points. Levels of mRNA were determined by quantitative real-time PCR. The mesors of PPAR α and PPAR γ in ApoE-KO mice and ApoE-KO LD/DL mice were improved compared with those in C57 mice. The amplitudes of PPAR α and PPAR γ were enhanced and the amplitudes of ROR α were attenuated in ApoE-KO mice and ApoE-KO LD/DL mice compared with C57 mice. The acrophases of PPAR α and PPAR γ were advanced and the acrophase of RXR α was delayed in ApoE-KO LD/DL mice compared with C57 and ApoE-KO mice. The expression levels of PPAR α and PPAR γ in ApoE-KO LD/DL mice were increased compared with C57 mice. The mRNA levels of clock genes were normalized to GAPDH mRNA ( for each group at every time point).