Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis
Expression analysis of two genes. (a) Expression profile of Hbγ-ECS1 in different tissues. (b) Expression patterns and relative mRNA levels of Hbγ-ECS1 at different tapping times after Ethrel stimulation. (c) Expression patterns and relative mRNA levels of Hbγ-ECS1 and Hbγ-ECS2 in long-term flowing latex of RRIM600. The long-term flowing latex was divided into the following flow times: morning latex, afternoon latex, and next-day latex. (d) Expression patterns and relative mRNA levels of Hbγ-ECS1 and Hbγ-ECS2 after being subjected to three months of every tapping intensity in PR107 (this figure expressed the difference in three months). Tapping system: half spiral tapping every 3 days along with Ethrel stimulation (15 d). Quantitative RT-PCR analysis was carried out to determine the expression levels. The 18SrRNA (GenBank Accession Number: AB268099) was used as the control. Data are expressed as mean ± SD for three individual experiments ().
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