Alendronate Can Improve Bone Alterations in Experimental Diabetes by Preventing Antiosteogenic, Antichondrogenic, and Proadipocytic Effects of AGEs on Bone Marrow Progenitor Cells

Figure 4

Effect of AGEs, alendronate, and vitamin E on the intracellular production of reactive oxygen species (ROS). BMPC were obtained from nondiabetic untreated animals and cultured for 48 hours with 200 mg/mL of either control BSA or AGEs-BSA, with or without 10⁻⁸M alendronate, in the presence or absence of 50 μM vitamin E. ROS production was assessed by the Rhodamine123 method. Results are expressed as the mean ± SEM. versus control BSA; versus BSA ± Ale, vitE, or Ale-vitE.