Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method

Table 2

Experimental design with real value and predicted value of conversion rate of ICA.

Run	(pH)	(temperature, °C)	(concentration of substrate, mg/mL)	(conversion rate of ICA, %)
				Experimental	Predicted
1	4.0	30	0.30	89.26	88.14
2	5.0	40	0.65	93.90	93.23
3	5.0	40	0.65	92.99	93.23
4	5.0	40	1.24	95.32	93.92
5	3.3	40	0.65	88.40	91.15
6	6.0	30	1.00	75.38	77.75
7	6.0	50	0.30	83.67	80.79
8	4.0	50	0.30	96.15	92.78
9	4.0	30	1.00	89.71	91.59
10	5.0	57	0.65	75.44	80.28
11	4.0	50	1.00	96.67	93.85
12	5.0	40	0.65	92.43	93.23
13	6.7	40	0.65	70.76	69.43
14	5.0	40	0.65	93.04	93.23
15	6.0	30	0.30	81.84	83.66
16	6.0	50	1.00	72.37	72.49
17	5.0	23	0.65	84.21	80.79
18	5.0	40	0.06	95.18	98.00
19	5.0	40	0.65	94.07	93.23
20	5.0	40	0.65	93.17	93.23