Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris

Table 2

Effect of metal ion of recombinant glucoamylase (rGA) activity.

Name of metal	Relative activity of recombinant glucoamylase (rGA)
	Metal salt (1 mM)	Metal salt (5 mM)
Control (H2O)	1.00	1.00
Na^+	1.04	0.86
K^+	1.04	0.99
Ca^++	1.03	1.19
Mg^++	1.06	1.13
Fe^++	1.50	0.96
Zn^++	1.15	0.73
Cu^++	1.63	0.44
Pb^++	1.39	0.66
Cd^++	1.02	1.04

Having significant effect on activity of metal ion ( ≤ 0.05 by independent sample -test).