Microcystin Biosynthesis and mcyA Expression in Geographically Distinct Microcystis Strains under Different Nitrogen, Phosphorus, and Boron Regimes

Table 1

Primers used in this study.

Primers	Sequence	Description	Reference
mcyA-Cd 1F (forward)	5′-AAAATTAAAAGCCGTATCAAA-3′	mcyA (condensation domain)	[25]
mcyA-Cd 1R (reverse)	5′-AAAAGTGTTTTATTAGCGGCTCAT-3′
GM5F-GC-clamp	5′-GC-clamp-CCTACGGGAGGCAGCAG-3′	16S rRNA gene DGGE fragment amplification	[26]
786R (reverse)	5′-CTACCAGGGTATCTAATC-3′	16S rRNA amplification for real-time RT-PCR	[27]
27F (forward)	5′-AGAGTTTGATCMTGGCTCAG-3′	Complete sequence of 16S rRNA for nested-PCR	[28]
1525R (reverse)	5′-AAGGAGGTGATCCAGCC-3′	Complete sequence of 16S rRNA for nested-PCR	[28]
907R (reverse)	5′-CCGTCAATTCCTTTGAGTTT-3′	16S rRNA gene DGGE fragment amplification	[29]

GC-clamp: 5′-CGCCCGCCGCGCGCGCCGCCCGCCCCGCCCCCGACGGGGGG-3′.
GM5F (without GC-clamp) was used as forward primer for real-time RT-PCR.