Research Article

Microcystin Biosynthesis and mcyA Expression in Geographically Distinct Microcystis Strains under Different Nitrogen, Phosphorus, and Boron Regimes

Table 3

Phylogenetic affinity of 16S rRNA gene sequences retrieved from monthly cyanobacterial bloom samples.

Band numberNameAccession numberSimilarity (%)Taxonomic group

Durgakund Pond
1 Uncultured bacteriumJQ90601699Uncultured bacterium
2 Merismopedia glaucaAJ78104499Chroococcales, Cyanobacteria
3 Uncultured bacteriumKF418783ā€”Uncultured bacterium
4 Uncultured beta-proteobacteriumJN37163295Betaproteobacteria
5Porphyrobacter sp.AB29974999Sphingomonadales, Alphaproteobacteria
6Microcystis wesenbergiiAB66607999Chroococcales, Cyanobacteria
7Uncultured bacteriumHQ65365697Uncultured bacterium
8Microcystis aeruginosaAF13930499Chroococcales, Cyanobacteria
9Aquimonas sp.KF418784ā€”Xanthomonadales, Gammaproteobacteria
10Roseomonas sp.HM12437098Rhodospirillales, Alphaproteobacteria

Wangsong Reservoir
11Acinetobacter sp.KM10856379Pseudomonadales, Gammaproteobacteria
12Microcystis aeruginosaKF37257298Chroococcales, Cyanobacteria
13Microcystis aeruginosaKJ746519100Chroococcales, Cyanobacteria
14Malikia spinosaNR11422899Burkholderiales, Betaproteobacteria
15Aeromonas sp.KM36322999Aeromonadales, Gammaproteobacteria
16Rhodobacter sp.AB25140893Rhodobacterales, Alphaproteobacteria
17Uncultured alpha-proteobacteriumHM15367598Alphaproteobacteria
18Roseomonas lacusJQ34904799Rhodospirillales, Alphaproteobacteria
19Uncultured alpha-proteobacteriumHM153673100Alphaproteobacteria

All the bands except 3 and 9 were based on published papers; therefore bands 3 and 9 were submitted to Genbank (BankIt1645319 Seq1 KF418783 for DGGE band #3; BankIt1646633 Seq1 KF418784 for DGGE band #9).