Research Article
Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific Oyster Crassostrea gigas
Table 1
Primers used for CaM cloning and expression analysis.
| | Primer name | Sequence () | Size (nt) | Application |
| | 3 sites Adaptor Primer | CTGATCTAGAGGTACCGGATCC | 22 | 3′ RACE | | Oligo dT-3 sites Adaptor Primer | CTGATCTAGAGGTACCGGATCC(T)n | 22+n | 3′ RACE | | GSP-3′ RACE | GATGCTGATGGAAATGGAAC | 20 | 3′ RACE | | GSP1 | GGCTTCTCTCATCTCCTCTT | 20 | 5′ RACE | | GSP2 | CTTCTCTCATCTCCTCTTCG | 19 | 5′ RACE | | GSP3 | TTCTCTCATCTCCTCTTCGG | 19 | 5′ RACE | | AUAP | GGCCACGCGTCGACTAGTAC | 20 | 5′ RACE | | AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 38 | 5′ RACE | | CaM-F | GATGCTGATGGAAATGGAAC | 20 | For CaM gene in real-time PCR | | CaM-R | TTCACTCACTTCTTCCTCTG | 20 | For CaM gene in real-time PCR | | eLF1-F | ACCACCCTGGTGAGATCAAG | 20 | For internal reference gene in real-time PCR | | eLF1-R | ACGACGATCGCATTTCTCTT | 20 | For internal reference gene in real-time PCR |
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