MP Resulting in Autophagic Cell Death of Microglia through Zinc Changes against Spinal Cord Injury

Figure 5

MP induced autophagic cell death of microglia in vitro in LPS induced inflammation model. (a) After 12 hours of incubation, cell viability of microglia was measured in normal group, LPS group, LPS + 3-MA group, LPS + rapamycin group, LPS + MP group, LPS + MP + 3-MA group, and LPS + MP + TPEN group by MTT. MTT reductions were expressed as mean ± SD ( for each group). versus LPS group; versus LPS + MP group; versus normal group. (b), (c) After incubation as described above, the expression of LC-3B (b) and Beclin-1 (c) in microglia was assessed by western blot in normal group, LPS group, LPS + MP + 3-MA group, LPS + MP group, and LPS + MP + TPEN group. Relative amounts were expressed as mean ± SD ( for each group). versus normal group; , versus LPS + MP group; versus normal group.

(a)

(b)

(c)