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BioMed Research International
Volume 2016, Article ID 6171352, 7 pages
Research Article

The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

1Department of Physiology, Zhengzhou University, Zhengzhou, Henan 450001, China
2Stem Cell Research Center of Medical College, Zhengzhou University, Zhengzhou, Henan 450052, China
3Department of Pathophysiology, Zhengzhou University, Zhengzhou, Henan 450001, China

Received 10 May 2016; Revised 9 July 2016; Accepted 12 July 2016

Academic Editor: Oliver von Bohlen und Halbach

Copyright © 2016 Bo Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To investigate the effect of advanced motherhood on rat hippocampal neural stem cell proliferation. Methods. Female parents were subdivided into control and old mother group by age, and neural stem cells were cultured from hippocampal tissues for 24 h newborn offspring. The diameter and numbers of neurospheres were examined by microscopy, and differences in proliferation were examined by EdU immunofluorescence, CCK-8 assay, and cell cycle analysis. Results. The number of neurospheres in the old mother group after culture was lower than the control group. Additionally, neurospheres’ diameter was smaller than that of the control group (). The EdU positive rate of the old mother group was lower than that of the control group (). CCK-8 assay results showed that the absorbance values for the old mother group were lower than that of the control group at 48 h and 72 h (). The proportions of cells in the S and G2/M phases of the cell cycle for the older mother group were less than that found for the control group (). Conclusion. The proliferation rates of hippocampal NSCs seen in the older mother group were lower than that seen in the control group.