The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

Figure 3

Proliferation detection of two groups. EdU stains were used to analyze the proliferation of NSCs, which showed (control group (a), (b), and (c) and old mother group (d), (e), and (f); 200x magnification) that cells were displaying a blue fluorescence after Hoechst 33342 staining (a and d) and green fluorescence after Apollo staining (b and e). The combined image (c and f) revealed that the EdU positive rate of the old mother group was lower than the control group. versus the control group (g). CCK-8 assay revealed that the absorbance values of the old mother group was lower than that of the control group at 48 h and 72 h. versus the control group (h). .

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