TY - JOUR A2 - Hua, Lin AU - Kamen, Ali AU - Sun, Shanhui AU - Wan, Shaohua AU - Kluckner, Stefan AU - Chen, Terrence AU - Gigler, Alexander M. AU - Simon, Elfriede AU - Fleischer, Maximilian AU - Javed, Mehreen AU - Daali, Samira AU - Igressa, Alhadi AU - Charalampaki, Patra PY - 2016 DA - 2016/04/05 TI - Automatic Tissue Differentiation Based on Confocal Endomicroscopic Images for Intraoperative Guidance in Neurosurgery SP - 6183218 VL - 2016 AB - Diagnosis of tumor and definition of tumor borders intraoperatively using fast histopathology is often not sufficiently informative primarily due to tissue architecture alteration during sample preparation step. Confocal laser microscopy (CLE) provides microscopic information of tissue in real-time on cellular and subcellular levels, where tissue characterization is possible. One major challenge is to categorize these images reliably during the surgery as quickly as possible. To address this, we propose an automated tissue differentiation algorithm based on the machine learning concept. During a training phase, a large number of image frames with known tissue types are analyzed and the most discriminant image-based signatures for various tissue types are identified. During the procedure, the algorithm uses the learnt image features to assign a proper tissue type to the acquired image frame. We have verified this method on the example of two types of brain tumors: glioblastoma and meningioma. The algorithm was trained using 117 image sequences containing over 27 thousand images captured from more than 20 patients. We achieved an average cross validation accuracy of better than 83%. We believe this algorithm could be a useful component to an intraoperative pathology system for guiding the resection procedure based on cellular level information. SN - 2314-6133 UR - https://doi.org/10.1155/2016/6183218 DO - 10.1155/2016/6183218 JF - BioMed Research International PB - Hindawi Publishing Corporation KW - ER -