Discrepancies between Skinned Single Muscle Fibres and Whole Thigh Muscle Function Characteristics in Young and Elderly Human Subjects

Figure 2

Myosin heavy chain (MHC) separation. Electrophoresis was used for separation of MHC isoforms in the human VL muscle. The top-down method separating MHC isoforms as IIx, IIa, and I is illustrated in the graph. 1 and 3 indicate the MHC standard showing IIx and IIa and I, respectively. 2 indicates a human sample including only MHC I. Using this method, all samples of all subjects were found to be composed of those fibre MHC types.