Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILK/GSK-3β/Slug Signaling Pathway
Emodin repressed EMT by targeting ILK in A2780 and SK-OV-3 cells. (a, c) A2780 and SK-OV-3 cells were transfected with siRNA-ILK and treated with or without emodin 20 μM for 48 hours. Protein levels of ILK, p-GSK-3β, GSK-3β, β-catenin, Slug, E-cadherin, and N-cadherin were shown. (b, d) Quantitative analyses of factors in SK-OV-3 cells shown in (a) and (c). , , and . , . (e) Representative transwell migration and invasion assay of A2780 and SK-OV-3 cells after transfection with siRNA of ILK with or without emodin treatment. (f) Quantification of migration and invasion capacity of SK-OV-3 cells in (e). , , and . , . N.C. represents transfection of siRNA-control.