Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia

Figure 2

Mfn2 expression of trophoblastic cells in hypoxic conditions. TEV-1 cells were treated with 300 μM CoCl₂ for 0, 12, 24, 48, or 72 h. (a) Mfn2 mRNA levels in trophoblastic cells were determined using qRT-PCR. The results showed that CoCl₂ treatment reduced Mfn2 mRNA levels in trophoblastic cells as early as 12 h after treatment, and the reduction was time-dependent . (b) The western blot results also showed that the Mfn2 protein level of trophoblastic cells decreased in a time-dependent manner when exposed to CoCl₂, while the HIF-1α expression level increased . All data are presented as the mean ± SD of three independent experiments.

(a)

(b)