BioMed Research International / 2016 / Article / Fig 2

Research Article

Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-κB Signaling in Osteoarthritis

Figure 2

OPN promotes expression of MMP13 through NF-kappa B signaling. ((a)-(b)) The expression of OPN after OPN targeting siRNA in chondrocytes from mRNA expression (a) and protein levels (b). ((c)-(d)) The abundance of p65 (c) and phosphorylated p65 (d) after OPN treatment or OPN-siRNA treatment. (c) Immunoblotting of p65 (upper part) and quantification of relative p65 abundance (bottom) from data shown in upper part. (d) Immunoblotting of p-p65 (upper part) and quantification of relative p-p65 abundance (bottom) from data shown in upper part. (e) The translocation of p65 protein from the cytoplasm to the nucleus in indicated situations (100). ((f)-(g)) The protein abundance of MMP13 (f) and COL2A1 (g) in indicated situations. (f) Immunoblotting of MMP13 (bottom) and quantification of relative p65 abundance (upper part) from data shown in bottom. (g) Immunoblotting of COL2A1 (bottom) and quantification of relative p65 abundance (upper part) from data shown in bottom. Data are representative of two independent experiments with 4–6 repeats per group. indicates a statistically significant difference between two groups (). OPN: osteopontin and MMP13: matrix metalloproteinase 13.
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