Purification and Characterization of a Thermostable β-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases

Table 4

Hydrolytic activity of the purified enzyme on different polysaccharides.

Substrate (0.5%, w/v)	Relative activity (%)
Konjac glucomannan	100
Locust bean gum	88.15 ± 1.8
Carob galactomannan	91.85 ± 1.7
Guar galactomannan	35.70 ± 0.6
Ivory nut mannan	32.74 ± 0.3
1,4-Beta-D-mannan	46.22 ± 0.4
Wheat arabinoxylan	0
Beechwood xylan	0
Carboxymethyl cellulose	0

Assays were carried out at 65°C at pH 6.0 for 10 min in 0.05 mol/L citric acid-0.1 mol/L Na₂HPO₄ buffer.
Data are mean ± SD, .