Genetic Analysis of the Atrial Natriuretic Peptide Gene Polymorphisms among Essential Hypertensive Patients in Malaysia

Table 1

Oligonucleotides for amplification and screening of polymorphisms.

ANP gene polymorphism	Forward and reverse primer	PCR cycling conditions	PCR product (bp)	Reference
A191G	FP 5-AGAGGGGAACCAGAGAGGAACCAG-3; FP 5-CCATCAGGTCTGCGTTGGATAC-3	5 min 95°C; 60 sec 55°C; 90 sec 72°C; 60 sec 94°C; ×45; 10 sec 72°C; 4°C	189 and 197	Kato et al., [26]
I/D	FP 5-GCAGTCCAGCCTAGGTGATA-3; RP 5-TCCGGAGTAGCTAGGACTTACA-3	5 min 95°C; 60 sec 55°C; 90 sec 72°C; 60 sec 94°C; ×45; 10 sec 72°C; 4°C	230 and 238	Kato et al., [26]

PCR cycling conditions are represented as temperature and time of initial denaturation, denaturation, annealing, extension, and final extension × number of cycles.