BioMed Research International / 2016 / Article / Tab 1

Research Article

Genetic Analysis of the Atrial Natriuretic Peptide Gene Polymorphisms among Essential Hypertensive Patients in Malaysia

Table 1

Oligonucleotides for amplification and screening of polymorphisms.

ANP gene polymorphismForward and reverse primerPCR cycling conditionsPCR product (bp)Reference

A191GFP 5-AGAGGGGAACCAGAGAGGAACCAG-3
FP 5-CCATCAGGTCTGCGTTGGATAC-3
5 min 95°C
60 sec 55°C
90 sec 72°C
60 sec 94°C
×45
10 sec 72°C
4°C
189 and 197 Kato et al., [26]

I/DFP 5-GCAGTCCAGCCTAGGTGATA-3
RP 5-TCCGGAGTAGCTAGGACTTACA-3
5 min 95°C
60 sec 55°C
90 sec 72°C
60 sec 94°C
×45
10 sec 72°C
4°C
230 and 238 Kato et al., [26]

PCR cycling conditions are represented as temperature and time of initial denaturation, denaturation, annealing, extension, and final extension × number of cycles.

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