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BioMed Research International
Volume 2016 (2016), Article ID 6715295, 8 pages
Research Article

Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model

1Department of New Materials, Oscotec Inc., Seongnam-si 13488, Republic of Korea
2Department of Periodontology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea
3Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea

Received 13 October 2015; Revised 31 December 2015; Accepted 1 February 2016

Academic Editor: Costantino Del Gaudio

Copyright © 2016 Jin Wook Hwang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Absorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BMs can be rapidly degraded by enzymes in vivo. In a previous study, to delay degradation time, collagen fibers were treated with cross-linking agents. These compounds prevented the enzymatic degradation of BMs. However, cross-linked BMs can exhibit delayed tissue integration. In addition, the remaining cross-linker could induce inflammation. Here, we attempted to overcome these problems using a natural ECM membrane. The membrane consisted of freshly harvested porcine pericardium that was stripped from cells and immunoreagents by a cleaning process. Acellular porcine pericardium (APP) showed a bilayer structure with a smooth upper surface and a significantly coarser bottom layer. APP is an ECM with a thin layer (0.18–0.35 mm) but with excellent mechanical properties. Tensile strength of APP was  MPa. In in vivo experiments, APP was transplanted into rabbit tibia. The biocompatible material was retained for up to 3 months without the need for cross-linking. Therefore, we conclude that APP could support osteogenesis as a BM for up to 3 months.