BioMed Research International / 2016 / Article / Tab 2

Research Article

Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

Table 2

Comparison of GICA with some published HIV-1 p24 assays in recent years.

AssaySupportp24 detection limitExecution time

Colloidal gold immunochromatographic assay (GICA) Commercial assays Lateral flow test strip25 pg/mL20 min
Abbott ARCHITECT HIV Ag/Ab Combo (Chemiluminescentmicroparticle immunoassay) ARCHITECT automated analyzer18.4 pg/mL (SFTS standard)26 min [11]
Roche Elecsys® HIV combi (Chemiluminescentmicroparticle immunoassay)Modular automated analyzer0.9 IU/mL (WHO standard)18 min [12]
Biomérieux VIDAS HIV DUO Ultra (Enzyme linked fluorescence assay)VIDAS automated analyzer11.5 pg/mL (SFTS standard)120 min [13]
Bio-Rad GS HIV Combo Ag/Ab (Enzyme linked immunosorbent assay)Bio-Rad EVOLIS Automated System12.7 pg/mL (SFTS standard)>120 min [14]
Bio-Rad Genscreen ULTRA HIV Ag-Ab (Enzyme linked immunosorbent assay)Microplate13 pg/mL (SFTS standard)120 min [13]
Laboratory assays
 Ultrasensitive capacitive immunosensor assayCapacitive immunosensor system7.9 × 10−8 pg/mL20 min [15]
 Amperometricimmunosensor assay based on direct gold electroplating-modified electrodeAmperometricimmunosensor system8 pg/mL20 min [16]
 Amperometricimmunosensor assay based on acetone-extracted propolisAmperometricimmunosensor system6.4 pg/mL10 min [17]
 Boosted ELISA based on immune complex dissociation and amplified signalMicroplate0.5 pg/mL>120 min [18]
 Nanoparticle-based biobarcode amplification assayNanosphereVerigene ID Reader0.1 pg/mL >120 min [19]
 Magnetic immuno-chromatography assayMagnaBiosciences magnetic assay reader30 pg/mL40 min [20]

SFTS standard: French national reference of HIV-1 p24 Ag obtained from the French Society of Blood Transfusion.

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