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Assay | Support | p24 detection limit | Execution time |
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Colloidal gold immunochromatographic assay (GICA) Commercial assays | Lateral flow test strip | 25 pg/mL | 20 min |
Abbott ARCHITECT HIV Ag/Ab Combo (Chemiluminescentmicroparticle immunoassay) | ARCHITECT automated analyzer | 18.4 pg/mL (SFTS standard) | 26 min [11] |
Roche Elecsys® HIV combi (Chemiluminescentmicroparticle immunoassay) | Modular automated analyzer | 0.9 IU/mL (WHO standard) | 18 min [12] |
Biomérieux VIDAS HIV DUO Ultra (Enzyme linked fluorescence assay) | VIDAS automated analyzer | 11.5 pg/mL (SFTS standard) | 120 min [13] |
Bio-Rad GS HIV Combo Ag/Ab (Enzyme linked immunosorbent assay) | Bio-Rad EVOLIS™ Automated System | 12.7 pg/mL (SFTS standard) | >120 min [14] |
Bio-Rad Genscreen™ ULTRA HIV Ag-Ab (Enzyme linked immunosorbent assay) | Microplate | 13 pg/mL (SFTS standard) | 120 min [13] |
Laboratory assays | | | |
Ultrasensitive capacitive immunosensor assay | Capacitive immunosensor system | 7.9 × 10−8 pg/mL | 20 min [15] |
Amperometricimmunosensor assay based on direct gold electroplating-modified electrode | Amperometricimmunosensor system | 8 pg/mL | 20 min [16] |
Amperometricimmunosensor assay based on acetone-extracted propolis | Amperometricimmunosensor system | 6.4 pg/mL | 10 min [17] |
Boosted ELISA based on immune complex dissociation and amplified signal | Microplate | 0.5 pg/mL | >120 min [18] |
Nanoparticle-based biobarcode amplification assay | NanosphereVerigene ID Reader | 0.1 pg/mL | >120 min [19] |
Magnetic immuno-chromatography assay | MagnaBiosciences magnetic assay reader | 30 pg/mL | 40 min [20] |
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