Skin-Derived Precursors against UVB-Induced Apoptosis via Bcl-2 and Nrf2 Upregulation

Figure 6

Effect of UVB irradiation on cell senescence and DNA damage by SA-βgal and pH2AX analysis. (a) Immunohistochemical analysis of SA-βgal-positive and pH2AX positive expression in SKPs and FBs after UVB irradiation (magnification ×400). +: UVB irradiation; −: non-UVB irradiation. In the SA-βgal staining, senescent cells were stained blue-green with no stain in nuclei, while DNA-damaged cells display brown nuclei in pH2AX analysis. (b) Quantitative analysis of SA-βgal⁺ cells and pH2AX⁺ cells in four groups. SKPs⁺: SKPs irradiated group; SKPs⁻: SKPs unirradiated group; FBs⁺: FBs irradiated group; FBs⁻: FBs unirradiated group. ; ns: no significance.

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