Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform

Figure 4

Time-course images of iPSC-derived EB-explant outgrowth cells in automated culture. (a) Scheme for automated culture of EB-explant outgrowth cells. ((b), (c), (d), (e)) Phase-contrast photomicrography of day 1 (b), day 4 (c), day 8 (d), and day 23 (e) after the start of the automated culture. The EBs adhered to the culture surface (on day 1) and the cell outgrowth was clearly confirmed surrounding the EBs (on day 4), after which we performed the subculture manipulation (on day 7). The uniform cell distribution after passage could be confirmed by an image on day 8. The cells were cultured until reaching subconfluent state (on day 23). The experiments were repeated three independent times. The scale bars indicate 500 μm.

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