Is Hydrogen Peroxide a Suitable Apoptosis Inducer for All Cell Types?

Table 1

Primers for quantitative real-time PCR.

	Gene	Forward	Reverse	Thermocycle
F/C	Caspase-9	5′TCAGACATCGTATCCTCCA	5′AGTCACAGCAGCACA	98/3 min + 40 × [9/15 s; 61/40 s]
	P53	5′ACCTGCACTTACTCCCCGGT	5′TCTTATAGACGGCCACGGCG
	NF-B	5′AGGACTTAAAATGGCAGGAGAG	5′GCTGTTCGTAGTGGTAAGTCTG
	RIP1	5′GAACTAGGCTTCAGCAACTCCG	5′GCAGCCAAAGAGGGCTTTGG
	β-actin	5′GGCACCCAGCACAATGAAG	5′CCGATCCACACGGAGTACTTG
293T	Caspase-9	5′TGCTGAGCAGCGAGCTGTT	5′AGCCTGCCCGCTGGAT	98/3 min + 40 × [9/15 s; 61/40 s]
	P53	5′CCACCATCCACTACAACTACAT	5′CAAACACGGACAGGACCC
	NF-B	5′TCTCCCTGGTCACCAAGGAC	5′TCATAGAAGCCATCCCGGC
	RIP1	5′CATGGAAAAGGCGTGATACAC	5′ACTTCCCTCAGCTCATTGTG
	β-actin	5′GGCACCCAGCACAATGAAG	5′CCGATCCACACGGAGTACTTG

F/C: fibroblasts/cardiomyocytes.