Magnesium Attenuates Phosphate-Induced Deregulation of a MicroRNA Signature and Prevents Modulation of Smad1 and Osterix during the Course of Vascular Calcification

Figure 1

Magnesium prevents Pi-induced mineral deposition in HAVSMC. HAVSMC were cultured for 14 days at the indicated concentrations of Mg²⁺, 1.5 and 2 mM (Mg 1.5 and Mg 2, resp.), and in the presence of 3 mM Pi (Pi). (a) Alizarin red staining pictures, to allow visualization of calcium deposition, are representative of the respective conditions. (b) Von Kossa staining pictures, to allow visualization of calcium deposition, are representative of the respective conditions. (c) Calcium deposition from HAVSMC of the three different donors was assessed using OCP with increasing concentration of Mg²⁺ and induction of calcification by Pi. Data are represented as a ratio of Pi and are expressed as mean ± SEM for each condition (). Pi 3 mM condition was set to 1. Statistics were realised with ANOVA multigroup analysis using Fisher PSLD posttest. The control condition (Ct) is significantly different from all conditions. versus Ct and versus Pi.

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