Magnesium Attenuates Phosphate-Induced Deregulation of a MicroRNA Signature and Prevents Modulation of Smad1 and Osterix during the Course of Vascular Calcification

Figure 4

The expression of col1A1 and Runx2 mRNA is not markedly induced by Pi. Pi-induced HAVSMC were assessed at day 3 and day 10. Total RNA was extracted and reverse-transcribed, and expression of col1A1 (a) and Runx2 (b) was analyzed by q-PCR and compared to control cells treated with basal medium. The control condition was set to 1 (dashed line). The cumulated relative quantity (RQ) of the three different donors of HAVSMC is represented as a ratio of the control condition and expressed as mean ± SEM for each condition (). GAPDH was used as an internal reference gene. Statistics were realised with the Mann-Whitney test. versus Ct and versus Ct.

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(b)