Magnesium Attenuates Phosphate-Induced Deregulation of a MicroRNA Signature and Prevents Modulation of Smad1 and Osterix during the Course of Vascular Calcification

Table 2

Primers used for q-PCR were published previously or designed through the use of PrimerBank [30, 31, 42].

Gene name	Forward (5′-3′)	Reverse (5′-3′)	GenBank
GAPDH	ATGGAAATCCCATCACCATCTT	CGCCCCACTTGATTTTGG	NM_002046
β-actin	CCTCACCCTGAAGTACCCCA	TGCCAGATTTTCTCCATGTCC	NM_001101
Collagen 1A1	ACGAAGACATCCCACCAATCAC	TCATCGCACAACACCTTGC	NM_000088
Osterix	CCTCTGCGGGACTCAACAAC	AGCCCATTAGTGCTTGTAAAGG	NM_152860
Runx2	AGCTTCTGTCTGTGCCTTCTGG	GGAGTAGAGAGGCAAGAGTTT	NM_001024630
Smad1	TTCCATGCCTCCTCCACAAG	AGGCATTCGGCATACACCTC	NM_005900