Research Article

ChSte7 Is Required for Vegetative Growth and Various Plant Infection Processes in Colletotrichum higginsianum

Figure 3

Targeted disruption and complementation of ChSte7 gene. (a) ChSte7 locus and gene deletion vector. (b) PCR analysis of wild-type strain (Ch-1), ChSte7 disruption mutants (ΔChSte7-26 and ΔChSte7-48), and complementation strain (CΔChSte7-26-20). Markers (M) in the top and bottom images are DL2000 and DL5000, respectively. The band of 877 bp in hph gene was amplified in the ChSte7 disruption mutants and complementation strain while a 1208 bp fragment in ChSte7 gene was obtained in the wild-type and complementation strains. (c) RT-PCR analysis of wild-type strain, ChSte7 disruption mutants, and complementation strain.
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