BioMed Research International / 2016 / Article / Tab 1

Research Article

Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing

Table 1

Testing matrix.

Challenge microorganismBiofilm growth medium (BGM)ModelTest methodologyDressings tested (Table 2)

Antibiotic-resistant Pseudomonas aeruginosa NCTC 13437

Community-acquired Methicillin-resistant Staphylococcus aureus (CA-MRSA) USA300

Candida albicans NCPF 3179
 Foetal calf serum (FCS)  Simple biofilm modelElemental analysis (K+, Mg2+, Ca2+, Zn2+, Ag+) of residual biofilm after dressing being applied for 24 hours (a simple and chemically consistent model to investigate formulation effects) ()CMC
P. aeruginosa NCTC 8506
Elemental analysis (Ag+) and quantitative live/dead staining after dressing being applied for 48 hours (a more challenging strain and longer dressing exposure time selected to challenge the silver-containing dressings) ()NGAD

S. aureus (clinical wound isolate, CI72) and Klebsiella pneumoniae (clinical isolate, CI45)TSB : FCS (50 : 50)Simulated wound polymicrobial biofilm modelQuantitative microbiology (total viable counts), PNA FISH, live/dead and Calcofluor White staining after dressing application for 48 hours (a more complex inoculum and longer dressing exposure time selected to challenge the silver dressings) ()NGAD