Research Article
Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing
Table 3
Reproducibility of the biofilm models utilised, as analysed by different analytical, imaging, and microbiological assays.
| Model | Strain | Analysis method | | Average | Std Dev |
| Simple biofilm model | Antibiotic-resistant P. aeruginosa NCTC 8506 | Elemental analysis (values minus blank filter disc elemental analysis) | 24 | 26.8 µM Mg2+ | 3.2 µM | 36.2 µM K+ | 7.1 µM | 171.9 µM Ca2+ | 27.0 µM | Antibiotic-resistant P. aeruginosa NCTC 13437 | Live/dead image analysis by pixilation | 19 | 25.6 µm thick | 8.2 µm |
| Simulated wound polymicrobial biofilm model | S. aureus, K. pneumoniae, 24-hour control | Confocal imaging of maximum depth | 15 | 9.74 µm thick | 2.37 µm | Total viable counts | 3 | 9.4 × 108 cfu S. aureus | 2.0 × 108 cfu | 7.7 × 109 cfu K. pneumoniae | 3.2 × 109 cfu |
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