Diagnosis of Human Axillary Osmidrosis by Genotyping of the Human ABCC11 Gene: Clinical Practice and Basic Scientific Evidence

Figure 1

Genotyping at ABCC11 538G>A by the SmartAmp method. (a) Flowchart of the SmartAmp-based genotyping. After a simple heat treatment to degrade RNA and denature proteins, blood samples were added to the reaction mixture (total 25 μL) and subjected to isothermal incubation at 60°C for 30 min while the fluorescence intensity was monitored. (b) Detection of the SNP 538G>A in ABCC11 by the SmartAmp method. Time-dependent increases in fluorescence intensity produced by the SmartAmp reaction with ABCC11 allele-specific primers carrying 538G (WT) or 538A (SNP) alleles were monitored by a real-time PCR system (Mx3000P; Stratagene). (c) Schematic illustration of the human ABCC11 gene and relative positions of each primer for SmartAmp-based genotyping. The details of the DNA amplification process were described in Aw et al. [19]. (d) Schematic illustration for multiple end-point detection of SmartAmp-based SNP typing with a CCD camera-linked digital processor.

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